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In Vitro and In Vivo Models for the Development of Hair Growth Materials By Regulating the β -Catenin Signaling Pathways.

Min Jeong WooJeongIn ChoiHa Yeong KangSehyeon JangMin Jeong KimSeaHyun KimSanguine ByunSung Keun Jung
Published in: Journal of medicinal food (2024)
Although hair loss contributes to various social and economic, research methods for material development are currently limited. In this study, we established a research model for developing materials for hair growth through the regulation of β -catenin. We confirmed that 100 nM tegatrabetan (TG), a β -catenin inhibitor, decreased the proliferation of human hair follicle dermal papilla cells (HFDPCs) at 72 h. In addition, TG-induced apoptosis suppressed the phosphorylation of GSK-3 β and Akt, translocation of β -catenin from the cytosol to the nucleus, and the expression of cyclin D1. Interestingly, TG significantly increased the G2/M arrest in HFDPCs. Subcutaneous injection of TG suppressed hair growth and the number of hair follicles in C57BL/6 mice. Moreover, TG inhibited the expression of cyclin D1, β -catenin, keratin 14, and Ki67. These results suggest that TG-induced inhibition of hair growth can be a promising model for developing new materials for enhancing β -catenin-mediated hair growth.
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