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FlsnRNA-seq: protoplasting-free full-length single-nucleus RNA profiling in plants.

Yanping LongZhijian LiuJinbu JiaWeipeng MoLiang FangDongdong LuBo LiuHong ZhangWei ChenJixian Zhai
Published in: Genome biology (2021)
The broad application of single-cell RNA profiling in plants has been hindered by the prerequisite of protoplasting that requires digesting the cell walls from different types of plant tissues. Here, we present a protoplasting-free approach, flsnRNA-seq, for large-scale full-length RNA profiling at a single-nucleus level in plants using isolated nuclei. Combined with 10x Genomics and Nanopore long-read sequencing, we validate the robustness of this approach in Arabidopsis root cells and the developing endosperm. Sequencing results demonstrate that it allows for uncovering alternative splicing and polyadenylation-related RNA isoform information at the single-cell level, which facilitates characterizing cell identities.
Keyphrases
  • single cell
  • rna seq
  • high throughput
  • nucleic acid
  • induced apoptosis
  • gene expression
  • healthcare
  • stem cells
  • cell death
  • bone marrow
  • cell therapy