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A loop-mediated isothermal amplification method for rapid direct detection and differentiation of nonpathogenic and verocytotoxigenic Escherichia coli in beef and bovine faeces.

A Ch StratakosM LintonS MillingtonIrene R Grant
Published in: Journal of applied microbiology (2017)
The multiplex LAMP assay represents a rapid alternative to culture for monitoring E. coli levels on beef for hygiene monitoring purposes, and, potentially, a method for detection of VTEC in beef and faeces.
Keyphrases
  • loop mediated isothermal amplification
  • escherichia coli
  • sensitive detection
  • high throughput
  • quantum dots
  • biofilm formation
  • staphylococcus aureus
  • pseudomonas aeruginosa
  • real time pcr
  • oral health