Comparative analysis of human, rodent and snake deltavirus replication.
Pierre KhalfiZoé DenisJoe McKellarGiovanni MerollaCarine ChaveyJosé Ursic-BedoyaLena SoppaLeonora SziroviczaUdo HetzelJeremy DufourtCedric LeyratNora GoldmannKaku GotoEloi VerrierThomas F BaumertDieter GlebeValérie CourgnaudDamien GregoireJussi HepojokiKarim MajzoubPublished in: PLoS pathogens (2024)
The recent discovery of Hepatitis D (HDV)-like viruses across a wide range of taxa led to the establishment of the Kolmioviridae family. Recent studies suggest that kolmiovirids can be satellites of viruses other than Hepatitis B virus (HBV), challenging the strict HBV/HDV-association dogma. Studying whether kolmiovirids are able to replicate in any animal cell they enter is essential to assess their zoonotic potential. Here, we compared replication of three kolmiovirids: HDV, rodent (RDeV) and snake (SDeV) deltavirus in vitro and in vivo. We show that SDeV has the narrowest and RDeV the broadest host cell range. High resolution imaging of cells persistently replicating these viruses revealed nuclear viral hubs with a peculiar RNA-protein organization. Finally, in vivo hydrodynamic delivery of viral replicons showed that both HDV and RDeV, but not SDeV, efficiently replicate in mouse liver, forming massive nuclear viral hubs. Our comparative analysis lays the foundation for the discovery of specific host factors controlling Kolmioviridae host-shifting.
Keyphrases
- hepatitis b virus
- high resolution
- single cell
- sars cov
- liver failure
- small molecule
- endothelial cells
- cell therapy
- high throughput
- induced apoptosis
- stem cells
- protein protein
- oxidative stress
- photodynamic therapy
- cell proliferation
- high speed
- mesenchymal stem cells
- signaling pathway
- climate change
- fluorescence imaging