HPLC of Monolayer-Protected Gold Clusters with Baseline Separation.
Stefan KnoppePascal VogtPublished in: Analytical chemistry (2019)
The properties of ultrasmall metal nanoparticles (ca. 10-200 metal atoms), or monolayer-protected metal clusters (MPCs), drastically depend on their atomic structure. For systematic characterization and application, assessment of their purity is of high importance. Currently, the gold standard for purity control of MPCs is mass spectrometry (MS). Mass spectrometry, however, cannot always detect small impurities; MS of certain clusters, for example, ESI-TOF of Au40(SR)24, is not successful at all. We here present a simple reversed-phase HPLC method for purity control of a series of small alkanethiolate-protected gold clusters. The method allows the detection of small impurities with high sensitivity. Linear correlation between alkyl chain length of Au25(SC nH2 n+1)18 clusters ( n = 6, 8, 10, 12) and their retention time was noticed.
Keyphrases
- mass spectrometry
- ms ms
- liquid chromatography
- high performance liquid chromatography
- gas chromatography
- capillary electrophoresis
- high resolution
- tandem mass spectrometry
- simultaneous determination
- sensitive detection
- multiple sclerosis
- solid phase extraction
- silver nanoparticles
- ionic liquid
- room temperature
- real time pcr
- walled carbon nanotubes