A genetically encoded fluorescent biosensor for extracellular L-lactate.
Yusuke NasuCiaran Murphy-RoyalYurong WenJordan N HaideyRosana S MolinaAbhi AggarwalShuce ZhangYuki KamijoMarie-Eve PaquetKaspar PodgorskiMikhail DrobizhevJaideep S BainsMary Joanne LemieuxGrant R GordonRobert E CampbellPublished in: Nature communications (2021)
L-Lactate, traditionally considered a metabolic waste product, is increasingly recognized as an important intercellular energy currency in mammals. To enable investigations of the emerging roles of intercellular shuttling of L-lactate, we now report an intensiometric green fluorescent genetically encoded biosensor for extracellular L-lactate. This biosensor, designated eLACCO1.1, enables cellular resolution imaging of extracellular L-lactate in cultured mammalian cells and brain tissue.