Single-cell transcriptome atlas reveals protective characteristics of COVID-19 mRNA vaccine.
Yong TanShuaiyao LuBo WangXuewen DuanYunkai ZhangXiaozhong PengHangwen LiAng LinZhenzhen ZhanXingguang LiuPublished in: Journal of medical virology (2022)
Messenger RNA (mRNA) vaccines are promising alternatives to conventional vaccines in many aspects. We previously developed a lipopolyplex (LPP)-based mRNA vaccine (SW0123) that demonstrated robust immunogenicity and strong protective capacity against severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) infection in mice and rhesus macaques. However, the immune profiles and mechanisms of pulmonary protection induced by SW0123 remain unclear. Through high-resolution single-cell analysis, we found that SW0123 vaccination effectively suppressed SARS-CoV-2-induced inflammatory responses by inhibiting the recruitment of proinflammatory macrophages and increasing the frequency of polymorphonuclear myeloid-derived suppressor cells. In addition, the apoptotic process in both lung epithelial and endothelial cells was significantly inhibited, which was proposed to be one major mechanism contributing to vaccine-induced lung protection. Cell-cell interaction in the lung compartment was also altered by vaccination. These data collectively unravel the mechanisms by which the SW0123 protects against lung damage caused by SARS-CoV-2 infection.
Keyphrases
- single cell
- respiratory syndrome coronavirus
- sars cov
- rna seq
- coronavirus disease
- high glucose
- high throughput
- endothelial cells
- diabetic rats
- high resolution
- induced apoptosis
- signaling pathway
- binding protein
- pulmonary hypertension
- cell death
- oxidative stress
- mesenchymal stem cells
- mass spectrometry
- type diabetes
- cell therapy
- drug induced
- gene expression
- skeletal muscle
- adipose tissue
- anti inflammatory
- tandem mass spectrometry
- data analysis
- nucleic acid