Rapid Detection of Ethambutol-Resistant Mycobacterium tuberculosis from Sputum by High-Resolution Melting Analysis in Beijing, China.

Jun WangWeijie ZhaoRongmei LiuFengmin HuoLingling DongYi XueYufeng WangZhongtan XueLiping MaYu Pang

Published in: Infection and drug resistance (2020)

MeltPro assay is a promising diagnostic tool for the detection of EMB resistance in China. The specific amino acid substitution in embB gene is the major reason for discrepancies between MeltPro assay and phenotypic DST.

Keyphrases

mycobacterium tuberculosis
high resolution
amino acid
high throughput
pulmonary tuberculosis
cystic fibrosis
air pollution
mass spectrometry
copy number
gene expression
dna methylation
loop mediated isothermal amplification
label free
quantum dots
high speed
data analysis