Potential of Autologous Progenitor Cells and Decellularized Porcine Artery Matrix in Construction of Tissue-engineered Vascular Grafts.
Jieh-Neng WangChung-Dann KanShao-Hsien LinKo-Chi ChangStephanie TsaoTak-Wah WongPublished in: Organogenesis (2021)
To develop a tissue-engineered vascular graft, we used pericardial effusion-derived progenitor cells (PEPCs) collected from drained fluid after open-heart surgery in children with congenital heart diseases to repopulate a decellularized porcine pulmonary artery. The PEPCs were compared with human fibroblasts (HS68) and human umbilical vein endothelial cells (HUVECs) in cell growth and migration. They were cultured with the matrices via an inner approach (intima), lateral approach (media), and outer approach (adventitia). PEPCs grew and migrated better than the other two cells 14 days after seeding in the decellularized vessel. In immunofluorescence assays, PEPCs expressed CD90 and CD105 indicating a vascular differentiation. PEPCs grew in a decellularized porcine pulmonary artery matrix may have the potential for producing tissue-engineered vascular grafts.
Keyphrases
- pulmonary artery
- endothelial cells
- extracellular matrix
- coronary artery
- pulmonary hypertension
- pulmonary arterial hypertension
- minimally invasive
- tissue engineering
- heart failure
- induced apoptosis
- high glucose
- young adults
- type diabetes
- bone marrow
- cell cycle arrest
- cardiovascular disease
- vascular endothelial growth factor
- coronary artery bypass
- endoplasmic reticulum stress
- platelet rich plasma