Evaluation of a ddPCR Commercial Assay for the Absolute Quantification of the Monkeypox Virus West Africa in Clinical Samples.

Elena PomariAntonio MoriSilvia AccordiniAnnalisa DoniniMaddalena CordioliEvelina TacconelliConcetta Castilletti

Published in: Diagnostics (Basel, Switzerland) (2023)

Overall, our data showed that the commercial ddPCR assay allowed the DNA detection of MPXV in 87.5% (35/40) of our cohort, highlighting useful technical indications for the different specimens with a potential greatest performance for skin lesions and rectal swabs.

Keyphrases

high throughput
circulating tumor
electronic health record
loop mediated isothermal amplification
soft tissue
single molecule
big data
cell free
real time pcr
human health
deep learning
risk assessment
nucleic acid
disease virus