Chromosome-specific painting in Thinopyrum species using bulked oligonucleotides.

Chen ChenYangshuo HanHe XiaoBingcan ZouDandan WuLina ShaCairong YangSongqing LiuYiran ChengYi WangHouyang KangXing FanYonghong ZhouTao ZhangHaiqin Zhang

Published in: TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik (2023)

. All 14 pairs of chromosomes of tetraploid Th. elongatum have maintained complete synteny with those of diploid Th. elongatum (Thy14), but the two sets of E genomes have diverged. This study also demonstrated that the E genome-specific painting probes are useful for rapid and effective detection of the alien genetic material of E genome in wheat-Thinopyrum derived lines.

Keyphrases

genome wide
loop mediated isothermal amplification
copy number
small molecule
dna methylation
nucleic acid
fluorescence imaging
gene expression
real time pcr