3D Imaging and Quantification of the Integrin at a Single-Cell Base on a Multisignal Nanoprobe and Synchrotron Radiation Soft X-ray Tomography Microscopy.
Chunyu ZhangShengkun YaoChao XuYa-Nan ChangYunbing ZongKai ZhangXiangzhi ZhangLijuan ZhangChunying ChenYu-Liang ZhaoHuaidong JiangXueyun GaoYaling WangPublished in: Analytical chemistry (2020)
The development of three-dimensional (3D) single-cell imaging and protein quantitative methods can provide more comprehensive information for diagnoses. We report the design and synthesis of a multisignal nanoprobe (AuGdNC@BSA-CV) for single-cell 3D imaging and quantifying the integrin αIIbβ3 using correlated synchrotron radiation soft X-ray tomography microscopy and an iterative tomographic algorithm termed equally sloped tomography for the first time. Moreover, on the basis of the Au or Gd content of our nanoprobe, the number of integrin αIIbβ3 on a single cell also can be accurately quantified (1.5 × 107 per cell) via inductively coupled plasma mass spectrometry.
Keyphrases
- single cell
- high resolution
- rna seq
- mass spectrometry
- high throughput
- high speed
- living cells
- tandem mass spectrometry
- cell adhesion
- single molecule
- computed tomography
- deep learning
- machine learning
- cell therapy
- radiation induced
- high performance liquid chromatography
- health information
- quantum dots
- photodynamic therapy