High-depth fluorescence imaging using a two-photon FRET system for mitochondrial pH in live cells and tissues.

Min Jung ChangKyutae KimKyun Seob ParkJi Su KangChang Su LimHwan Myung KimChulhun KangMin Hee Lee

Published in: Chemical communications (Cambridge, England) (2018)

We developed a fluorescent pH probe (1) capable of two-photon excitation and far-visible-emission based on FRET, composed of naphthalimide-piperazine-rhodamine. It exhibited a pH-dependent reversible and fast ratiometric fluorescence change in the rhodamine emission. Probe 1 was applied to image the pH perturbations of mitochondria in living cells and tissues.

Keyphrases

living cells
fluorescent probe
fluorescence imaging
single molecule
gene expression
induced apoptosis
cell cycle arrest
energy transfer
cell death
deep learning
optical coherence tomography
cell proliferation
nitric oxide
endoplasmic reticulum