Dual fluorescent labeling of GLP-1R in live cells via enzymatic tagging and bioorthogonal chemistry.

Tracey M LewandowskiPeng An Carlo P Ramil Ming Fang Qing Lin

Published in: RSC chemical biology (2022)

To study GPCR conformational dynamics in live cells, here we report an integrated approach combining enzymatic SNAP-tagging with bioorthogonal chemistry for dual fluorescent labeling of GLP-1R. The resulting GLP-1R conformational biosensors permit a FRET-based analysis of the receptor subdomain movement in response to ligand stimulation in live cells.

Keyphrases

induced apoptosis
cell cycle arrest
single molecule
living cells
quantum dots
hydrogen peroxide
molecular dynamics simulations
cell death
cell proliferation
fluorescent probe
drug discovery