A rapid and non-destructive method for spatial-temporal quantification of colonization by Pseudomonas syringae pv. tomato DC3000 in Arabidopsis and tomato.

We present a non-destructive method to quantify colonisation by bioluminescent Pst::LUX in plants. Using a common Gel Doc system and imaging software, our method requires less time and labour than conventional methods that are based on destructive sampling of infected leaf material. Furthermore, in contrast to conventional strategies, our method provides additional information about the spatial-temporal patterns of Pst colonisation.