Molecular Pro-Apoptotic Activities of Flavanone Derivatives in Cyclodextrin Complexes: New Implications for Anticancer Therapy.
Angelika A Adamus-GrabickaPawel HikiszArtur StępniakMagdalena MaleckaPiotr PanethJoanna SikoraElzbieta BudziszPublished in: International journal of molecular sciences (2024)
This study evaluates the antiproliferative potential of flavanones, chromanones and their spiro-1-pyrazoline derivatives as well as their inclusion complexes. The main goal was to determine the biological basis of molecular pro-apoptotic activities and the participation of reactive oxygen species (ROS) in shaping the cytotoxic properties of the tested conjugates. For this purpose, changes in mitochondrial potential and the necrotic/apoptotic cell fraction were analyzed. Testing with specific fluorescent probes found that ROS generation had a significant contribution to the biological anticancer activity of complexes of flavanone analogues. TT (thrombin time), PT (prothrombin time) and APTT (activated partial tromboplastin time) were used to evaluate the influence of the compounds on the extrinsic and intrinsic coagulation pathway. Hemolysis assays and microscopy studies were conducted to determine the effect of the compounds on RBCs.
Keyphrases
- cell death
- reactive oxygen species
- anti inflammatory
- single molecule
- structure activity relationship
- living cells
- high throughput
- dna damage
- high resolution
- small molecule
- single cell
- human health
- cell therapy
- oxidative stress
- label free
- physical activity
- quantum dots
- optical coherence tomography
- bone marrow
- risk assessment
- mesenchymal stem cells
- ionic liquid
- cancer therapy
- climate change
- high speed
- fluorescence imaging
- replacement therapy
- molecular dynamics simulations