Antibiotic Resistance and Genotypes of Nosocomial Strains of Acinetobacter baumannii in Kazakhstan.
Alyona V LavrinenkoEugene A SheckSvetlana KolesnichenkoIlya S AzizovAnar TurmukhambetovaPublished in: Antibiotics (Basel, Switzerland) (2021)
The aim of this study was to determine the prevalence of A. baumannii antibiotic-resistant strains in Kazakhstan and to characterize genotypes related to epidemic "high-risk" clones. Two hundred and twenty four A. baumannii isolates from four cities of Kazakhstan in 2011-2019 were studied. Antibiotic susceptibility testing was performed by using broth microdilutions method according to EUCAST (v 11.0) recommendations. The presence of blaOXA-23-like, blaOXA-24/40-like,blaOXA-58-like,blaVIM,blaIMP, and blaNDM genes was determined by PCR. Genotyping was performed using high-throughput real-time PCR detection of 21 SNPs at 10 chromosomal loci used in existing MLST schemes. Resistance rates to imipenem, meropenem, amikacin, gentamicin, and ciprofloxacin were 81.3%, 78.6%, 79.9%, 65.2%, and 89.3%, respectively. No colistin resistant isolates were detected. The values of the MIC 50% and the MIC 90% of tigecycline were 0.125 mg/L, only four isolates (1.8%) had the ECOFF value >0.5 mg/L. The presence of acquired carbapenemase genes was found in 82.2% strains, including blaOXA-23-like (78.6%) or blaOXA-58-like (3.6%) genes. The spreading of carbapenem resistant A. baumannii strains in Kazakhstan was associated with epidemic "high-risk" clonal groups, predominantly, CG208(92)OXF/CG2PAS (80.8%) and less often CG231(109)OXF/CG1PAS (1.8%).
Keyphrases
- acinetobacter baumannii
- genome wide
- real time pcr
- pseudomonas aeruginosa
- multidrug resistant
- drug resistant
- escherichia coli
- high throughput
- klebsiella pneumoniae
- dna methylation
- gram negative
- copy number
- cystic fibrosis
- genome wide identification
- bioinformatics analysis
- genetic diversity
- risk factors
- gene expression
- genome wide analysis
- genome wide association
- clinical practice
- drug induced