FK506-binding protein 5 regulates cell quiescence-proliferation decision in zebrafish epithelium.
Yingxiang LiChengdong LiuXuanxuan BaiMingyu LiCunming DuanPublished in: FEBS letters (2023)
Using a zebrafish ionocyte model, transcriptomics and genetic analyses were performed to identify pathways and genes involved in cell quiescence-proliferation regulation. GO and KEGG pathway analyses revealed that genes involved in transcription regulation, cell cycle, Foxo signaling, and Wnt signaling pathway are enriched among the up-regulated genes, while those involved in ion transport, cell adhesion, and oxidation-reduction are enriched among the down-regulated genes. Among the top up-regulated genes is FK506-binding protein 5 (Fkbp5). Genetic deletion and pharmacological inhibition of Fkbp5 abolished ionocyte reactivation and impaired Akt signaling. Forced expression of a constitutively active form of Akt rescued the defects caused by Fkbp5 inhibition. These results uncover a key role of Fbkp5 in regulating the quiescence-proliferation decision via Akt signaling.
Keyphrases
- signaling pathway
- binding protein
- cell cycle
- single cell
- genome wide
- pi k akt
- cell proliferation
- transcription factor
- induced apoptosis
- cell adhesion
- epithelial mesenchymal transition
- genome wide identification
- dna methylation
- bioinformatics analysis
- cell therapy
- decision making
- stem cells
- hydrogen peroxide
- nitric oxide
- gene expression
- endoplasmic reticulum stress