Bactericidal Effect of 5-Mercapto-2-nitrobenzoic Acid-Coated Silver Nanoclusters against Multidrug-Resistant Neisseria gonorrhoeae.
María Isabel LucíoMaria-Eleni KyriaziJoshua HamiltonDiego BatistaAlexander SheppardElisabeth Sams-DoddMaria Victoria HumbertIrshad HussainMyron ChristodoulidesAntonios G KanarasPublished in: ACS applied materials & interfaces (2020)
Neisseria gonorrhoeae is among the most multidrug-resistant bacteria in circulation today, and new treatments are urgently needed. In this work, we demonstrate the ability of 5-mercapto-2-nitrobenzoic acid-coated silver nanoclusters (MNBA-AgNCs) to kill strains of Neisseria gonorrhoeae. Using an in vitro bactericidal assay, MNBA-AgNCs had been found to show significantly higher anti-gonococcal bioactivity than the antibiotics ceftriaxone and azithromycin and silver nitrate. These nanoclusters were effective against both planktonic bacteria and a gonococcal infection of human cell cultures in vitro. Treatment of human cells in vitro with MNBA-AgNCs did not induce significant release of lactate dehydrogenase, suggesting minimal cytotoxicity to eukaryotic cells. Our results suggest that MNBA-AgNCs hold great potential for topical treatment of localized gonorrhoeae.
Keyphrases
- multidrug resistant
- gold nanoparticles
- sensitive detection
- drug resistant
- escherichia coli
- endothelial cells
- silver nanoparticles
- fluorescent probe
- induced apoptosis
- nitric oxide
- single cell
- cell therapy
- cell death
- cell cycle arrest
- mesenchymal stem cells
- combination therapy
- stem cells
- signaling pathway
- risk assessment
- replacement therapy
- endoplasmic reticulum stress
- smoking cessation