Imidazolium-based mass tags for protein biomarker detection using laser desorption ionization mass spectrometry.
Mingxia LiuJinjuan XueHu-Wei LiuYu BaiPublished in: Chemical communications (Cambridge, England) (2023)
Novel imidazolium-based mass tags (IMTs) were designed, synthesized and applied to simultaneous in situ analysis of multiple biomarkers on less than 10 cells. The high sensitivity, flexible extensibility and excellent distinguishability of IMTs open new avenues for designing common mass tag templates suitable for mass spectrometric immunoassay and provide an ideal option for multiplex-sensitive detection at the cellular scale.
Keyphrases
- sensitive detection
- ionic liquid
- loop mediated isothermal amplification
- mass spectrometry
- quantum dots
- induced apoptosis
- real time pcr
- cell cycle arrest
- minimally invasive
- high throughput
- high resolution
- cell death
- protein protein
- oxidative stress
- high performance liquid chromatography
- small molecule
- signaling pathway
- pi k akt
- gas chromatography
- binding protein