A Multiplex Assay for Fast PIK3CA Hotspot Mutation Characterization in a Single Specimen by 3-Color Digital PCR Analysis.
Jean HelmijrGianmarco MottaLisa JongbloedVanja de WeerdLotte van BergenNoortje VerschoorStefania StellaCorine BeaufortPaolo VigneriJohn W M MartensSaskia M WiltingMaurice P H M JansenPublished in: The journal of applied laboratory medicine (2024)
Our designed multiplex dPCR assay detected PIK3CA mutations with high accuracy in stock and pre-amplified cfDNA. Furthermore, it is affordable and demands less cfDNA input when compared to available uniplex dPCR assays and NGS analyses.