Precise correlative method of Cryo-SXT and Cryo-FM for organelle identification.
Haobo BaiYong GuanJianhong LiuLiang ChenWenbin WeiGang LiuYangchao TianPublished in: Journal of synchrotron radiation (2020)
Cryogenic soft X-ray tomography (Cryo-SXT) is ideally suitable to image the 3D sub-cellular architecture and organization of cells with high resolution in the near-native preservation state. Cryogenic fluorescence microscopy (Cryo-FM) can determine the location of a molecule of interest that has been labeled with a fluorescent tag, thus revealing the function of the cells. To understand the relations between the sub-cellular architecture and the function of cells, correlative Cryo-SXT and Cryo-FM was applied. This method required the matching of images of different modalities, and the accuracy of the matching is important. Here, a precise correlative method of Cryo-SXT and Cryo-FM is introduced. The capability of matching images of different modalities with high resolution was verified by simulations and practical experiments, and the method was used to identify vacuoles and mitochondria.
Keyphrases
- electron microscopy
- high resolution
- induced apoptosis
- cell cycle arrest
- mass spectrometry
- deep learning
- optical coherence tomography
- oxidative stress
- high speed
- signaling pathway
- convolutional neural network
- endoplasmic reticulum stress
- magnetic resonance imaging
- machine learning
- cell proliferation
- computed tomography
- high throughput
- pi k akt