A highly sensitive ratiometric fluorescence immunoassay based on bioorthogonal nanozymes.

Xiajian LiuNianhua WangYixuan HouHe DongWenhua LiangXinchun LiYouyong Yuan

Published in: Chemical communications (Cambridge, England) (2024)

We designed a novel ratiometric fluorescence immunoassay based on bioorthogonal nanozymes for carcinoembryonic antigen detection. The analytical performance of our designed immunoassay showed a wide linear range, a low detection limit, good reproducibility, selectivity and stability. Thus, bioorthogonal nanozymes hold great potential applications in clinical diagnoses.

Keyphrases

sensitive detection
label free
loop mediated isothermal amplification
fluorescent probe
energy transfer
living cells
quantum dots
single molecule
real time pcr
molecularly imprinted
tandem mass spectrometry