Echinococcus granulosus is the etiologic agent of cystic echinococcosis. Numerous research studies have been conducted on natural scolicidal agents to inactivate protoscolices during surgery. This study was undertaken to compare the in vitro scolicidal effects of hydroalcoholic extracts of Calendula officinalis, Artemisia dracunculus, Artemisia absinthium and Ferula assafoetida. The scolicidal activities of the extracts were tested at different concentrations following incubation periods of 10, 30 and 60 min. The chemical composition of the hydroalcoholic extracts were analyzed using Gas Chromatography-Mass Spectrometry (GC-MS). The major chemical components of C. officinalis, A. dracunculus, A. absinthium and F. assafoetida were identified as n-Docosane (14.17%), 2H-1-benzopyran-2-one, 7-methoxy (54.96%), n-Docosane (9.72%) and 2-methoxy-3-methyl-butyric acid, methyl ester (13.9%), respectively. The results showed that the hydroalcoholic extracts of A. absinthium and F. assafoetida at a concentration of 250 mg/ml resulted in killing 100% of the protoscolices at 60 minutes, while the concentration of 250 mg/ml of hydroalcoholic extract of C. officinalis and A. dracunculus resulted in killing 42.33% and 65.67%, respectively. The findings of the present study showed that A. absinthium and F. assafoetida have potent scolicidal effects. However, additional in vivo studies are required to confirm the efficacy of these plant-derived extracts against hydatid cyst for their clinical use.