Electrochemical sensor for rapid determination of fibroblast growth factor receptor 4 in raw cancer cell lysates.
Rebeca M Torrente-RodríguezVíctor Ruiz-Valdepeñas MontielSusana CampuzanoMaría PedreroMeryem FarchadoEva VargasF Javier Manuel de VillenaMaría Garranzo-AsensioRodrigo BarderasJosé Manuel PingarrónPublished in: PloS one (2017)
The first electrochemical immunosensor for the determination of fibroblast growth factor receptor 4 (FGFR4) biomarker is reported in this work. The biosensor involves a sandwich configuration with covalent immobilization of a specific capture antibody onto activated carboxylic-modified magnetic microcarriers (HOOC-MBs) and amperometric detection at disposable carbon screen-printed electrodes (SPCEs). The biosensor exhibits a great analytical performance regarding selectivity for the target protein and a low LOD of 48.2 pg mL-1. The electrochemical platform was successfully applied for the determination of FGFR4 in different cancer cell lysates without any apparent matrix effect after a simple sample dilution and using only 2.5 μg of the raw lysate. Comparison of the results with those provided by a commercial ELISA kit shows competitive advantages by using the developed immunosensor in terms of simplicity, analysis time, and portability and cost-affordability of the required instrumentation for the accurate determination of FGFR4 in cell lysates.
Keyphrases
- label free
- molecularly imprinted
- solid phase extraction
- gold nanoparticles
- sensitive detection
- liquid chromatography tandem mass spectrometry
- loop mediated isothermal amplification
- liquid chromatography
- high throughput
- ionic liquid
- mass spectrometry
- magnetic resonance imaging
- stem cells
- cell therapy
- nitric oxide
- gas chromatography
- binding protein
- protein protein
- tandem mass spectrometry
- small molecule
- low cost
- diffusion weighted imaging