Detection of HbD Punjab Trait in Saurashtra Region of Gujarat by HPLC Method.
Sagar DholariyaDeepak N ParchwaniAmit SonagraRagini SinghAnita MotianiMehul KaliyaGarima AnandaniPublished in: Indian journal of clinical biochemistry : IJCB (2023)
The HbD Punjab trait is a less common hemoglobin variant in the Saurashtra region of Gujarat. This patient presented to the General Medicine and Biochemistry Department at All India Institute of Medical Sciences, Rajkot, Hospital, for the HbA1 c analysis by high-performance liquid chromatography (HPLC). The patient was 52 years old, a known case of type 2 diabetes mellitus and hypertension, and presented with complaints of generalized malaise and fatigability for the previous ten to twelve months. On physical examination, there was no evidence of pallor, lymphadenopathy, splenomegaly, or hepatomegaly. Peripheral blood smear revealed normocytic normochromic RBC, and platelets were adequate and normal in morphology. The HPLC analysis revealed heterozygous for hemoglobin D (HbD Punjab trait), and false high HbA1c corresponds to fasting plasma glucose (FPG) levels due to abnormal separation. However, genetic counseling is highly recommended to detect any potential reproductive risk factors.
Keyphrases
- high performance liquid chromatography
- simultaneous determination
- ms ms
- solid phase extraction
- tandem mass spectrometry
- mass spectrometry
- genome wide
- risk factors
- peripheral blood
- liquid chromatography
- healthcare
- case report
- blood pressure
- mental health
- dna methylation
- insulin resistance
- physical activity
- early onset
- gene expression
- weight loss
- tertiary care
- adipose tissue
- skeletal muscle
- hiv testing
- real time pcr
- arterial hypertension