Biocompatible and Photostable Photoacoustic Contrast Agents as Nanoparticles Based on Bodipy Scaffold and Polylactide Polymers: Synthesis, Formulation, and In Vivo Evaluation.
Jean-Baptiste BodinJérôme GateauJustine CoïsThéotim LucasFlora LefebvreLaurence MoineMagali NoirayCatherine CailleauStéphanie DenisGilles ClavierNicolas TsapisRachel Méallet-RenaultPublished in: ACS applied materials & interfaces (2022)
We have designed a new Bodipy scaffold for efficient in vivo photoacoustic (PA) imaging of nanoparticles commonly used as drug nanovectors. The new dye has an optimized absorption band in the near-infrared window in biological tissue and a low fluorescence quantum yield that leads to a good photoacoustic generation efficiency. After Bodipy-initiated ring-opening polymerization of lactide, the polylactide-Bodipy was formulated into PEGylated nanoparticles (NPs) by mixing with PLA-PEG at different concentrations. Formulated NPs around 100 nm exhibit excellent PA properties: an absorption band at 760 nm and a molar absorption coefficient in between that of molecular PA absorbers and gold NPs. Highly improved photostability compared to cyanine-labeled PLA NPs as well as innocuity in cultured macrophages were demonstrated. After intravenous injection in healthy animals, NPs were easily detected using a commercial PA imaging system and spectral unmixing, opening the way to their use as theranostic agents.
Keyphrases
- fluorescence imaging
- fluorescent probe
- living cells
- photodynamic therapy
- oxide nanoparticles
- high resolution
- single molecule
- drug delivery
- magnetic resonance
- magnetic resonance imaging
- molecular dynamics
- endothelial cells
- emergency department
- walled carbon nanotubes
- low dose
- energy transfer
- computed tomography
- ionic liquid
- mass spectrometry
- highly efficient
- contrast enhanced
- electronic health record
- light emitting
- positron emission tomography
- dual energy