DNA-Conjugated Gold Nanoparticles as High-Mass Probes in Imaging Mass Cytometry.
Brian MalileJelena BrkicAlexandre BouzekriDerek J WilsonOlga OrnatskyChun PengJennifer I-Ling ChenPublished in: ACS applied bio materials (2019)
We employ imaging mass cytometry (IMC) to investigate in vitro uptake and cellular distribution of DNA-functionalized gold nanoparticles (AuNPs). IMC enables the multiparametric imaging of cell components and allows for the detection of AuNPs in cells with >100 times higher sensitivity than conventional confocal fluorescence imaging, as each nanoparticle contains thousands of atoms for signal amplification. Changes in the accumulation of nanoparticles in cells due to oligonucleotide sequence-dependent interactions are exploited to examine a model biomarker for hypoxia-microRNA-210. We find that AuNPs functionalized with microRNA-210-targeting sequence accumulate in hypoxic cells 3 to 4-fold compared to normoxic cells. The work examines the potential use of DNA-AuNP as high-mass probes for the analysis of nonabundant nucleic acids.
Keyphrases
- induced apoptosis
- fluorescence imaging
- gold nanoparticles
- cell cycle arrest
- high resolution
- single molecule
- single cell
- circulating tumor
- photodynamic therapy
- small molecule
- endoplasmic reticulum stress
- oxidative stress
- drug delivery
- cell proliferation
- signaling pathway
- cell therapy
- bone marrow
- endothelial cells
- mesenchymal stem cells
- cancer therapy
- pi k akt
- simultaneous determination
- circulating tumor cells