Prediction of the Adult T-Cell Leukemia Inhibitory Activity of Blueberry Leaves/Stems Using Direct-Injection Electron Ionization-Mass Spectrometry Metabolomics.
Hisahiro KaiYoshihito OkadaYo GotoTakayuki NakayamaKazuhiro SugamotoKenjirou OgawaMasao YamasakiKazuhiro MorishitaKoji MatsunoHisato KunitakePublished in: Plants (Basel, Switzerland) (2022)
Although Vaccinium virgatum Aiton leaves and stems inhibit adult T-cell leukemia (ATL) cells, leaves and stems can differ between individual plants and by time and location. In this study, leaf and stem components were profiled in the same individual plant using direct-injection electron ionization-mass spectrometry (DI-EI-MS) metabolomics, with the aims of analyzing the anti-ATL activity, and quantifying proanthocyanidins (PACs). Leaves, stems, and leaf/stem mixtures showed distinct and characteristic spectra. Anti-ATL activity was stronger in stems than leaves, and the PAC content was higher in stems than leaves. These data were subjected to bivariate analysis to identify the factor ( m/z ) responsible for the inhibitory effect of ATL based on the highest coefficient of determination ( R 2 ). The results of this DI-EI-MS metabolomics analysis suggest that among PACs contained in V. virgatum stems and leaves, the fragment ion at m/z 149 contributes significantly to anti-ATL activity.
Keyphrases
- mass spectrometry
- gas chromatography
- liquid chromatography
- essential oil
- high resolution
- capillary electrophoresis
- bone marrow
- induced apoptosis
- computed tomography
- magnetic resonance imaging
- oxidative stress
- young adults
- cystic fibrosis
- escherichia coli
- cell cycle arrest
- tandem mass spectrometry
- solid phase extraction
- endoplasmic reticulum stress
- molecularly imprinted
- simultaneous determination
- artificial intelligence
- big data
- contrast enhanced