High-resolution melting assay for rapid, simultaneous detection of JAK2 , MPL and CALR variants.

Christopher M SandeGuang YangAyman MohamedBen L LegendreDanielle PionStephanie L FerroKate GrimmKojo S J Elenitoba-Johnson

Published in: Journal of clinical pathology (2023)

These studies demonstrate the exquisite accuracy, sensitivity and specificity of the HRM-based HemeScreen MPN assay, which serves as a powerful, clinically applicable platform for rapid, simultaneous detection of clinically relevant, somatic disease variants.

Keyphrases

loop mediated isothermal amplification
high resolution
copy number
high throughput
sensitive detection
label free
mass spectrometry
dna methylation
genome wide
case control
single cell
quantum dots