Delays during PBMC isolation have a moderate effect on yield, but severly compromise cell viability.
Tanja GolkePatrick MucherPatricia SchmidtAstrid RadakovicsManuela ReplPhilipp HoferThomas PerkmannMartina FondiKlaus G SchmettererHelmuth HaslacherPublished in: Clinical chemistry and laboratory medicine (2022)
In conclusion, our findings suggest that while post-centrifugation delays ≥24 h might have only a minor effect on cell yield and purity, their impact on cell viability is substantial, even when CPT are used.
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