Epigenetic landscape of intestinal cell line HT29 cocultured with Lacticaseibacillus .
Anunay SinhaSanjeev KhoslaPublished in: Epigenomics (2024)
Aim: To investigate the changes in epigenetic landscape of HT29 cells upon coculture with the Lacticaseibacillus. Materials & methods: Histone and m6A mRNA modifications were examined by biochemical and NGS-based methods including western blotting, colorimetric assays, ChIP-Seq and direct mRNA sequencing. LC-MS was performed to identify Lacticaseibacillus secretome. Results: In cocultured HT29 cells global enrichment of H3K9ac and H3K4me3 and depletion of H3K9me3 mark was observed; mean genic positional signals showed depletion of H3K9ac and H3K4me3 at the TSS but enrichment in the upstream region; m6A methylation was altered in mRNAs corresponding to specific gene pathways; Lacticaseibacillus HU protein interacts with histone H3. Conclusion: Lacticaseibacillus can epigenetically alter specific genetic pathways in human intestinal cells.
Keyphrases
- induced apoptosis
- dna methylation
- cell cycle arrest
- genome wide
- single cell
- gene expression
- high throughput
- endoplasmic reticulum stress
- binding protein
- signaling pathway
- gold nanoparticles
- copy number
- oxidative stress
- nitric oxide
- sensitive detection
- hydrogen peroxide
- transcription factor
- induced pluripotent stem cells
- fluorescent probe
- genome wide identification