A FluoroSpot B assay for the detection of IgA and IgG SARS-CoV-2 spike-specific memory B cells: Optimization and qualification for use in COVID-19 vaccine trials.

Hélène BiscegliaJulie BarrierJoseline RuizAnke Pagnon

Published in: Journal of immunological methods (2023)

These results show that the dual-color IgA-IgG FluoroSpot provides a sensitive, specific, linear, and precise tool to detect spike-specific MBC responses. This MBC FluoroSpot assay is a method of choice for monitoring spike-specific IgA and IgG MBC responses induced by COVID-19 candidate vaccines in clinical trials.

Keyphrases

sars cov
coronavirus disease
clinical trial
respiratory syndrome coronavirus
working memory
study protocol
single cell
real time pcr