arfA antisense RNA regulates MscL excretory activity.
Rosa MorraFenryco PratamaThomas ButterfieldGeizecler TomazettoKate YoungRuth LopezNeil DixonPublished in: Life science alliance (2023)
Excretion of cytoplasmic protein (ECP) is a commonly observed phenomenon in bacteria, and this partial extracellular localisation of the intracellular proteome has been implicated in a variety of stress response mechanisms. In response to hypoosmotic shock and ribosome stalling in Escherichia coli , ECP is dependent upon the presence of the large-conductance mechanosensitive channel and the alternative ribosome-rescue factor A gene products. However, it is not known if a mechanistic link exists between the corresponding genes and the respective stress response pathways. Here, we report that the corresponding mscL and arfA genes are commonly co-located on the genomes of Gammaproteobacteria and display overlap in their respective 3' UTR and 3' CDS. We show this unusual genomic arrangement permits an antisense RNA-mediated regulatory control between mscL and arfA , and this modulates MscL excretory activity in E. coli These findings highlight a mechanistic link between osmotic, translational stress responses and ECP in E. coli , further elucidating the previously unknown regulatory function of arfA sRNA.
Keyphrases
- escherichia coli
- genome wide
- genome wide identification
- nucleic acid
- transcription factor
- copy number
- genome wide analysis
- quantum dots
- bioinformatics analysis
- protein protein
- reactive oxygen species
- biofilm formation
- klebsiella pneumoniae
- visible light
- staphylococcus aureus
- gene expression
- amino acid
- small molecule
- candida albicans
- quality control