Development of quantitative analytical method for volatile thiol compound with LC-ESI-MS as nonvolatile derivative by integrating a thiol-specific derivatization.
Jiangfeng ZhaoKengo SuzukiIwao OhtsuPublished in: Bioscience, biotechnology, and biochemistry (2022)
Generally, volatile thiols are hard to be measured with electrospray-ionization-type LC-MS due to the volatility. Therefore, we here evaluated the pretreatment of their S-bimanyl derivatization by monobromobimane to enable the detection as nonvolatile derivative. Consequently, we successfully developed the convenient and efficient method through the quantitative analysis of 2-furanmethanethiol (volatile thiol odorant of coffee aroma) in coffee bean.
Keyphrases
- gas chromatography
- mass spectrometry
- ms ms
- liquid chromatography
- tandem mass spectrometry
- high resolution
- high resolution mass spectrometry
- simultaneous determination
- high performance liquid chromatography
- gas chromatography mass spectrometry
- ultra high performance liquid chromatography
- solid phase extraction
- liquid chromatography tandem mass spectrometry
- loop mediated isothermal amplification
- water soluble
- label free
- sensitive detection