Histone proteoform analysis reveals epigenetic changes in adult mouse brown adipose tissue in response to cold stress.
Bethany C TaylorLoic H SteinthalMichelle DiasHari Krishna YalamanchiliScott A OchsnerGladys E ZapataNitesh R MehtaNeil J McKennaNicolas L YoungAlli M Nuotio-AntarPublished in: Epigenetics & chromatin (2024)
Our results reveal global epigenetically-regulated transcriptional "on" and "off" signals in murine BAT in response to varying degrees of chronic cold stimuli and establish a novel methodology to quantitatively study histones in BAT, allowing for direct comparisons to decipher mechanistic changes during the thermogenic response. Additionally, we make histone PTM and proteoform quantitation, RNA splicing, RRBS, and transcriptional footprint datasets available as a resource for future research.
Keyphrases
- dna methylation
- adipose tissue
- gene expression
- transcription factor
- genome wide
- mass spectrometry
- ms ms
- insulin resistance
- current status
- high fat diet
- single cell
- metabolic syndrome
- high performance liquid chromatography
- rna seq
- long noncoding rna
- tandem mass spectrometry
- oxidative stress
- high resolution
- drug induced
- simultaneous determination