The Aβ42 Peptide and IAPP Physically Interact in a Yeast-Based Assay.
Daniel V KachkinVeronika V LashkulNatalia A GorshenevaSergey A FedotovMaria S RubelYury O ChernoffAleksandr A RubelPublished in: International journal of molecular sciences (2023)
Numerous studies have demonstrated that people with type 2 diabetes mellitus (associated with IAPP peptide aggregation) show an increased incidence of Alzheimer's disease (associated with Aβ aggregation), but the mechanism responsible for this correlation is presently unknown. Here, we applied a yeast-based model to study the interactions of IAPP with PrP (associated with TSEs) and with the Aβ42 peptide. We demonstrated that fluorescently tagged IAPP forms detergent-resistant aggregates in yeast cells. Using the FRET approach, we showed that IAPP and Aβ aggregates co-localize and physically interact in yeast cells. We also showed that this interaction is specific and that there is no interaction between IAPP and PrP in the yeast system. Our data confirmed a direct physical interaction between IAPP and Aβ42 aggregates in a living cell. Based on these findings, we hypothesize that this interaction may play a crucial role in seeding Aβ42 aggregation in T2DM patients, thereby promoting the development of AD.
Keyphrases
- saccharomyces cerevisiae
- induced apoptosis
- cell cycle arrest
- cell wall
- end stage renal disease
- ejection fraction
- physical activity
- mental health
- chronic kidney disease
- newly diagnosed
- stem cells
- signaling pathway
- platelet rich plasma
- high throughput
- single molecule
- single cell
- type diabetes
- peritoneal dialysis
- machine learning
- adipose tissue
- skeletal muscle
- electronic health record
- patient reported outcomes
- mesenchymal stem cells
- deep learning