Tn-seq of the Candida glabrata reference strain CBS138 reveals epigenetic plasticity, structural variation, and intrinsic mechanisms of resistance to micafungin.

Timothy J NickelsAndrew P GaleAbigail A HarringtonWinston TimpKyle W Cunningham

Published in: bioRxiv : the preprint server for biology (2024)

transposon and profiled using Tn-seq. Numerous genes that regulate innate and acquired resistance to an important clinical antifungal were uncovered, including a pleiotropic drug resistance gene (PDR1) and a duplication of part of one chromosome. Compounds that target PDR1 and other genes may augment the potency of existing antifungals.

Keyphrases

genome wide
dna methylation
candida albicans
copy number
genome wide identification
immune response
gene expression
biofilm formation
rna seq
single cell
genome wide analysis
transcription factor
cystic fibrosis