Reversible dynamic optical sensing based on coumarin modified β-cyclodextrin for glutathione in living cells.

Zhixue LiuMengdi TianHeng ZhangYu Liu

Published in: Chemical communications (Cambridge, England) (2023)

Coumarin acting as an optical probe was modified on ethylenediamine β-cyclodextrin, which not only enhanced its molecular binding affinity to glutathione (GSH) by a reversible Michael addition, showing 113 times more affinity than that of coumarin itself, but also achieved dynamic real-time sensing of glutathione in living HeLa cells.

Keyphrases

fluorescent probe
living cells
capillary electrophoresis
cell cycle arrest
high resolution
induced apoptosis
high speed
mass spectrometry
cell death
cell proliferation
signaling pathway
binding protein
transcription factor