Small-animal PET imaging analysis with [18 F]FHBG in a mouse model of HSV1-tk gene expression in melanoma.
Onseok LeeJaeyoung KimChil Hwan OhPublished in: Experimental dermatology (2018)
The purpose of this study was to establish a small-animal model for molecular imaging and to acquire basic data on assessing the efficacy of candidate melanoma drugs using small-animal PET imaging analysis with [18 F]FHBG for herpes simplex virus 1-thymidine kinase (HSV1-tk) gene expression in a melanoma mouse model. The B16 melanoma cell line was transduced with a recombinant lentiviral vector containing the HSV1-tk gene and inoculated into the back skin of C57BL/6J mice. [18 F]FHBG PET imaging showed better contrast for HSV1-tk(+) melanomas compared to brain, heart, gall bladder, intestine and kidney than did [18 F]FDG PET imaging.
Keyphrases
- metabolic syndrome
- pet imaging
- herpes simplex virus
- gene expression
- mouse model
- positron emission tomography
- dna methylation
- skin cancer
- computed tomography
- magnetic resonance
- basal cell carcinoma
- type diabetes
- spinal cord injury
- genome wide
- multiple sclerosis
- machine learning
- copy number
- transcription factor
- drug induced
- artificial intelligence
- data analysis
- gene therapy