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Supercharging and multiple reaction monitoring of high-molecular-weight intact proteins using triple quadrupole mass spectrometry.

Durga D KhanalYehia Z BaghdadyBenjamin J FigardKevin A Schug
Published in: Rapid communications in mass spectrometry : RCM (2019)
The feasibility for top-down quantitative analysis of high-molecular-weight proteins with a triple quadrupole mass spectrometer was demonstrated. Further, additives such as TFE can be highly beneficial for increased chargeability and response of the proteins.
Keyphrases
  • mass spectrometry
  • liquid chromatography
  • high resolution
  • high performance liquid chromatography
  • gas chromatography
  • tandem mass spectrometry
  • simultaneous determination
  • capillary electrophoresis