The splicing factor hnRNPL demonstrates conserved myocardial regulation across species and is altered in heart failure.
Isabelle DraperWanting HuangSuchita PandeAaron ZouTimothy D CalamarasRichard H ChoeAna Correia-BrancoAriel L MeiHoward H ChenHannah R LittelMekala GunasekaranNatalya M WellsChristine C BruelsAudrey L DaughertyMatthew J WolfPeter B KangVicky K YangDonna K SlonimMary C WallingfordRobert M BlantonPublished in: FEBS letters (2024)
Heart failure (HF) is highly prevalent. Mechanisms underlying HF remain incompletely understood. Splicing factors (SF), which control pre-mRNA alternative splicing, regulate cardiac structure and function. This study investigated regulation of the splicing factor heterogeneous nuclear ribonucleoprotein-L (hnRNPL) in the failing heart. hnRNPL protein increased in left ventricular tissue from mice with transaortic constriction-induced HF and from HF patients. In left ventricular tissue, hnRNPL was detected predominantly in nuclei. Knockdown of the hnRNPL homolog Smooth in Drosophila induced cardiomyopathy. Computational analysis of predicted mouse and human hnRNPL binding sites suggested hnRNPL-mediated alternative splicing of tropomyosin, which was confirmed in C2C12 myoblasts. These findings identify hnRNPL as a sensor of cardiac dysfunction and suggest that disturbances of hnRNPL affect alternative splicing in HF.
Keyphrases
- left ventricular
- heart failure
- acute heart failure
- cardiac resynchronization therapy
- hypertrophic cardiomyopathy
- acute myocardial infarction
- high glucose
- end stage renal disease
- aortic stenosis
- endothelial cells
- mitral valve
- left atrial
- chronic kidney disease
- diabetic rats
- atrial fibrillation
- newly diagnosed
- oxidative stress
- type diabetes
- small molecule
- transcription factor
- high resolution
- peritoneal dialysis
- mass spectrometry
- acute coronary syndrome
- insulin resistance
- transcatheter aortic valve replacement
- aortic valve
- spinal cord
- skeletal muscle
- protein protein
- induced pluripotent stem cells