Determining transaminase activity in bacterial libraries by time-lapse imaging.
Carlos J C RodriguesJoão Miguel SanchesCarla C C R de CarvalhoPublished in: Chemical communications (Cambridge, England) (2019)
Transaminase activity was determined by time-lapse imaging using a colourimetric reaction and image analysis. A correlation between the benzaldehyde conversion and relative luminance was determined, allowing the identification of the most promising biocatalysts, the determination of kinetic parameters, and the assessment of the effect of the substrate concentration on activity.