Automated Image Analysis of Transmission Electron Micrographs: Nanoscale Evaluation of Radiation-Induced DNA Damage in the Context of Chromatin.

Mutaz A Abd Al-RazaqAnna IsermannMarkus Hecht Claudia E Rübe

Published in: Cells (2023)

The results of our image analysis suggest that high-LET IR induces chromatin relaxation along particle trajectories, enabling the critical repair of successive DNA damage. Following exposure to different radiation qualities, automated image analysis of nanoparticle-labeled DNA repair proteins in the chromatin ultrastructure enables precise characterization of specific DNA damage patterns.

Keyphrases

dna damage
dna repair
radiation induced
deep learning
oxidative stress
radiation therapy
machine learning
high throughput
dna damage response
depressive symptoms
computed tomography
gene expression
transcription factor
atomic force microscopy
high resolution
iron oxide