In-Depth Qualitative Analysis of Lime Essential Oils Using the Off-Line Combination of Normal Phase High Performance Liquid Chromatography and Comprehensive Two-Dimensional Gas Chromatography-Quadrupole Mass Spectrometry.
Mariosimone ZoccaliBarbara GiocastroIvana L BonaccorsiAlessandra TrozziPeter Q TranchidaLuigi MondelloPublished in: Foods (Basel, Switzerland) (2019)
The present research is focused on the in-depth qualitative analysis of three types of lime essential oil (EO), viz., Key (A and B) and Persian, using the off-line combination of normal phase high performance liquid chromatography (NP-HPLC) and comprehensive two-dimensional gas chromatography-quadrupole mass spectrometry (GC × GC-QMS). The first analytical dimension (NP-HPLC) was exploited for the isolation of the hydrocarbon constituents from the oxygenated ones. Each fraction was then reduced in volume and analyzed using (cryogenic modulation) GC × GC-QMS. Peak assignment was carried out through the combined use of mass spectral database and linear retention index matching processes. The powerful four-dimensional technology enabled the separation and identification of a very high number (153) of lime essential oil volatile compounds.
Keyphrases
- gas chromatography
- high performance liquid chromatography
- essential oil
- tandem mass spectrometry
- liquid chromatography
- mass spectrometry
- simultaneous determination
- solid phase extraction
- high resolution mass spectrometry
- optical coherence tomography
- gas chromatography mass spectrometry
- capillary electrophoresis
- high resolution
- systematic review
- computed tomography
- emergency department
- magnetic resonance imaging
- electronic health record
- neural network