Rational Design, Synthesis of Fluorescence Probes for Quantitative Detection of Amyloid-β in Alzheimer's Disease Based on Rhodamine-Metal Complex.
Jingjing XiangChunbai XiangLihua ZhouMengsi SunLixiong FengChuangjun LiuLintao CaiPing GongPublished in: Analytical chemistry (2022)
The efficient detection and monitoring of amyloid-β plaques (Aβ42) can greatly promote the diagnosis and therapy of Alzheimer's disease (AD). Fluorescence imaging is a promising method for this, but the accurate determination of Aβ42 still remains a challenge. The development of a reliable fluorescent probe to detect Aβ42 is essential. Herein, we report a rational design strategy for Aβ42 fluorescence probes based on rhodamine-copper complexes, Rho1-Cu-Rho4-Cu, among them Rho4-Cu exhibits the best performance including high sensitivity (detection limit = 24 nM), high affinity ( K d = 23.4 nM), and high selectivity; hence, Rho4-Cu is selected for imaging Aβ42 in AD mice, and the results showed that this probe can differentiate normal mice and AD mice effectively.
Keyphrases
- fluorescent probe
- living cells
- fluorescence imaging
- photodynamic therapy
- single molecule
- protein kinase
- high fat diet induced
- high resolution
- loop mediated isothermal amplification
- smooth muscle
- real time pcr
- label free
- aqueous solution
- metal organic framework
- small molecule
- metabolic syndrome
- type diabetes
- wild type
- energy transfer
- insulin resistance
- quantum dots
- adipose tissue
- bone marrow
- solid phase extraction
- replacement therapy
- nucleic acid