Carbonized Silk Nanofibers in Biodegradable, Flexible Temperature Sensors for Extracellular Environments.

Temperature is one of the key parameters for activity of cells. The trade-off between sensitivity and biocompatibility of cell temperature measurement is a challenge for temperature sensor development. Herein, a highly sensitive, biocompatible, and degradable temperature sensor was proposed to detect the living cell extracellular environments. Biocompatible silk materials were applied as sensing and packing layers, which endow the device with biocompatibility, biodegradability, and flexibility. The silk-based temperature sensor presented a sensitivity of 1.75%/°C and a working range of 35-63 °C with the capability to measure the extracellular environments. At the bending state, this sensor worked at promising response of cells at different temperatures. The applications of this developed silk material-based temperature sensor include biological electronic devices for cell manipulation, cell culture, and cellular metabolism.