Rapid ABO genotyping by high-speed droplet allele-specific PCR using crude samples.

Chiaki TairaKazuyuki MatsudaNaoya TakeichiSatomi FurukawaMitsutoshi SuganoTakeshi UeharaNobuo OkumuraTakayuki Honda

Published in: Journal of clinical laboratory analysis (2017)

The droplet-AS-PCR method enabled rapid and specific amplification of three SNPs of the ABO gene from crude DNA treated with proteinase K. ABO genotyping by the droplet-AS-PCR has the potential to be applied to various fields including a forensic medicine and transplantation medical care.

Keyphrases

high throughput
high speed
genome wide
single cell
atomic force microscopy
real time pcr
dna methylation
nucleic acid
loop mediated isothermal amplification
circulating tumor
cell free
high resolution
single molecule
genetic diversity
gene expression
risk assessment
newly diagnosed
quantum dots
sensitive detection