Propolis Reduces Phosphatidylcholine-Specific Phospholipase C Activity and Increases Annexin a7 Level in Oxidized-LDL-Stimulated Human Umbilical Vein Endothelial Cells.
Hongzhuan XuanZhen LiJiying WangKai WangChongluo FuJianlong YuanFuliang HuPublished in: Evidence-based complementary and alternative medicine : eCAM (2014)
To understand the mechanisms underlying the regulating dyslipidemia action of Chinese propolis and Brazilian green propolis, we investigated their effects on phosphatidylcholine-specific phospholipase C (PC-PLC) activity and annexin a7 (ANXA7) level which play crucial roles in the control of the progress of atherosclerosis. Furthermore, active oxygen species (ROS) levels, nuclear factor-KappaB p65 (NF- κ B p65), and mitochondrial membrane potential (MMP) were also investigated in oxidized-LDL- (ox-LDL-) stimulated human umbilical vein endothelial cells (HUVECs). Our data indicated that the treatment of both types of propolis 12.5 μ g/mL significantly increased cell viability and attenuated apoptosis rate, increased ANXA7 level, and decreased PC-PLC activity. Both types of propolis also inhibited ROS generation as well as the subsequent MMP collapse, and NF- κ B p65 activation induced by ox-LDL in HUVECs. Our results also indicated that Chinese propolis and Brazilian green propolis had similar biological activities and prevented ox-LDL induced cellular dysfunction in HUVECs.
Keyphrases
- low density lipoprotein
- nuclear factor
- endothelial cells
- oxidative stress
- high glucose
- cell death
- signaling pathway
- toll like receptor
- dna damage
- lps induced
- diabetic rats
- reactive oxygen species
- machine learning
- cell migration
- pi k akt
- mass spectrometry
- stress induced
- electronic health record
- big data
- deep learning
- artificial intelligence