Copaiba Oil-Based Emulsion as a Natural Chemotherapeutic Agent for the Treatment of Bovine Mastitis: In Vivo Studies.
Katieli da Silva Souza CampanholiRanulfo Combuca da Silva JuniorFlávia Amanda Pedroso de MoraisRenato Sonchini GonçalvesBruna Moura RodriguesMagali Soares Dos Santos PozzaLidiane Vizioli de Castro-HoshinoSilvio Mayke LeiteOtávio Augusto CapelotoMauro Luciano BaessoPaulo Cesar PozzaWilker CaetanoPublished in: Pharmaceutics (2023)
Copaiba oil-resin (COR) extracted from Copaifera reticulata Ducke has been used as a natural chemotherapeutic agent for a wide range of therapeutic applications. This study presents an emulgel design with a high concentration of COR, designed to prevent and treat mastitis. The COR was stabilized in a gel matrix constituted by carbopol C934P and Pluronic ® F127 (ECO formulation) ratios. The permeation study of ECO was accessed by Fourier transform infrared photoacoustic spectroscopy (FTIR-PAS). The results reveal a high capacity of ECO to permeate deep skin layers. Dairy cows with a history of mastitis were used as in vivo models and exposed to ECO treatment. Monitoring of the teat's inflammatory response showed that ECO effectively prevents mastitis. Furthermore, the ECO formulation was able to form a thin film gel on the application side, preventing fly proliferation and significantly reducing the pathogen load. This study reveals a drug that can used as an alternative application for mastitis in human or veterinary clinics.